For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets.
- Up to 100X higher fidelity than Taq polymerase
- Crude DNA sample will not inhibit amplification
- Ideal for cloning, mutagenesis, sequencing and microarrays
- Options for blunt end or A-overhang products
- Optimized buffers include PCR enhancers
Use our convenient PCR Selector Tool to determine the correct Accuris PCR Reagent for your applications.
Accuris High Fidelty Polymerase (2 tube formulation)
- Leaves a blunt end for blunt end vector cloning
- 50x fidelity of native Taq
- Ideal for complex targets up to 10kb
For applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit. Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples.
A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.
Each 200 unit kit includes:
- 1 tube of High Fidelity Polymerase (2 U/μl)
- 3 tubes of 5X buffer (PCR enhancers, 5mM dNTPs, & 15mm MgCl2)
Accuris High Fidelity Master Mix
- Leaves a blunt end for blunt end vector cloning
- 100x fidelity of native Taq
- Ideal for shorter, less complex targets
A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.
The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.
Accuris High Fidelity, Hot Start Master Mix
- Leaves an A-overhang for TA cloning
- 10x fidelity of native Taq
- Ideal for difficult, high GC content sequences up to 10kb
Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance.
The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.
Each mix includes:
- High Fidelity Polymerase
- Buffer [PCR Enhancers, 2mM dNTPs, 6mM MgCl2 , hot-start antibodies(optional)]
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